Idizer (M-110P; Microfluidics Corp) at 16,000 psi. Unbroken cells have been removed
Idizer (M-110P; Microfluidics Corp) at 16,000 psi. Unbroken cells have been removed by centrifugation (12,000 ?g; 10 min; four ). Membranes had been collected from the supernatant by ultracentrifugation (150,000…