Y and counterstaining with ethidium bromide. Immunostained cells with (A) anti-fibronectin antibody, (B) antiCD90 antibody, (C) anti-CD45 antibody, (D) anti-CD106 antibody, (E) anti-nestin, (F) anti-neurofilament 160 (NF-160) antibody, (G) anti-NF68 antibody and (H) anti-glial fibrilliary acidic protein antibody.occurred, almost certainly as a consequence of toxicity. This getting was also reported by Kaka et al. [9]. On the other hand, the optimum expression of O1, O4 and Oligo2 was achieved at dose of 25 ng/mL. The differentiation of BMSC into OLC at this dose was 71 , even though Kaka et al. [9] reported a 58 differentiation at T3 concentration of ten ng/mL. T3 increases morphological and functional maturation of postmitotic oligodendrocytes, as indicated by a welldeveloped network of branched processes and by the expression of myelin oligodendrocyte glycoprotein andglutamine synthetase [16]. The T3 deficiency modifications the distribution of oligodendrocyte/myelin markers during oligodendroglial differentiation in vitro [9]. Even so, our outcomes show that T3 at concentration of 25 ng/mL is far more effective than 10-ng/mL concentration. Kang et al. [17] have generated NPC by exposing human embryonic stem cells to several inducing agents in DMEM/F12 supplement then by subjecting them to growth things EGF, PDGF, bFGF and T3 (30 ng/mL). By this approach, they achieved 81 differentiation into OLC [17]. On the other hand,http://IBJ.90396-00-2 Formula pasteur.ac.irAbbaszadeh et al.Iran. Biomed. J., April120(A)Positive cells ( )one hundred 90 80 70 60 50 40 30 20 10* *(B)Viable cells ( )80 60 40 20 0 BMSC Groups DMSOFNNtNF68 NF160 GFAP Markers in useOOOligoFig. 2. Histograms of quantitative evaluation of viability and unique markers by immunocytochemistry at pre-induction and induction stages.Fmoc-L-Val-OH Data Sheet (A) The percentage in the viable cells in untreated and treated bone marrow stromal cells (BMSC) at pre-induction stage.PMID:33432591 The histogram shows the viable cells in untreated BMSC (handle) and also the DMSO-retinoic acid treated cells. The viability was higher inside the handle group in comparison to the other group. (B) Quantitative analysis of various markers by immunocytochemistry at pre-induction stage. Black columns shows untreated BMSC and light gray columns shows BMSC treated with dimethyl sulfoxideretinoic acid. The fibronectin (FN) shows a considerable distinction in each group. *indicates statistical significance in between BMSC and the other experimental groups (P0.001). Nt, nestin; NF, neurofilament; GFAP, glial fibrilliary acidic proteinthis outcome could possibly be as a result of the source of cells utilized for differentiation, which resulted inside a higher percentage of oligodendrocytes. The current study supports the outcomes of earlier investigation on the effect of T3 on the differentiation stem cells into OLC [18]. Our RT-PCR outcomes showed that OLC expressed PDGFR-, whilst untreated BMSC and NPC did not. It was first reported that PDGF could be the cause of proliferation and differentiation of oligodendrocyteL Neuro D NeuroD Nprogenitor cells. The PDGF and its receptors are broadly expressed in each embryonic and adult central nervous technique [19]. In preceding research, the expression of PDGF in OLC derived from distinct regions from the central nervous technique which include brain has been studied, however the expression on bone marrowderived OLC has not been studied [20]. Kaka et al. [9] could differentiate OLC from BMSC, but PDGFR gene expression was not evaluated. Our data showedA (A)N GAPDH GAPDH Oct4 OctBNPOL(B) BN GAPDH GAPDH PDGFR- PDGFR-BNPOL(C) CFig. 3.