Product Name :
Phospho-NF-kappaB p65 (S529) Recombinant Rabbit Monoclonal Antibody [SP07-00]
Predicted band size :
60 kDa
Observed band size :
65 kDa
Synonyms:
Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody MGC131774 antibody NF kappa B p65delta3 antibody NFKB3 antibody Nuclear Factor NF Kappa B p65 Subunit antibody Nuclear factor NF-kappa-B p65 subunit antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody OTTHUMP00000233473 antibody OTTHUMP00000233474 antibody 展开
Function :
Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp κB sequence in the immunoglobulin κ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser529 of Human NF-kB p65.
Species reactivity:
Human
Verify application:
WB, IHC-P, FC, IP
Coupling :
unconjugated
Clone number:
SP07-00
RRID:
AB_3069692
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃.37342-97-5 structure Avoid repeated freeze / thaw cycles.Price of 4-Chloropyrimidine-2-carbonitrile
Storage buffer solution:
1*TBS (pH7.PMID:33635749 4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 60 kDa
Positive control :
HeLa treated with 100nM Calyculin A and 20ng/mL TNF-α for 15 minutes cell lysate, human lung tissue, human lung squamous cell carcinoma tissue, Daudi.