Product Name :
Phospho-Histone H2A.X (S139) Recombinant Rabbit Monoclonal Antibody [SR33-09]
Predicted band size :
15 kDa
Observed band size :
15/20 kDa
Synonyms:
AW228881 antibody H2A histone family member X antibody H2A.FX antibody H2A.X antibody H2a/x antibody H2AFX antibody H2AX antibody H2AX histone antibody H2AX_HUMAN antibody Hist5.2ax antibody 展开
Function :
Histone H2A.X is a member of the Histone H2A family, which is involved in nucleosomal organization of chromatin. An important phosphorylated form is γH2AX (S139), which forms when double-strand breaks appear. In humans and other eukaryotes, the DNA is wrapped around histone octamers, consisting of core histones H2A, H2B, H3 and H4, to form chromatin. H2AX contributes to nucleosome-formation, chromatin-remodeling and DNA repair, and is also used in vitro as an assay for double-strand breaks in dsDNA.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser139 of Human Histone H2AX.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IHC-P
Coupling :
unconjugated
Clone number:
SR33-09
RRID:
AB_3069632
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.Formula of 1260381-44-9 Aliquot store at -20℃ or -80℃.5-Chloro-2,3-dimethylpyrazine structure Avoid repeated freeze / thaw cycles.PMID:33506687
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
Predicted band size: 15 kDa
Positive control :
HeLa treated with 20μM Etoposide for 2 hours whole cell lysate, HeLa treated with UV for 2 hours whole cell lysate, NIH/3T3 treated with 25μM Etoposide for 5 hours whole cell lysate, HepG2 cell lysate–treated with etoposide, rat brain tissue, mouse testis tissue, mouse brain tissue, mouse small intestine tissue, human colon carcinoma tissue.